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. 2018 Jan 31;8(1):170207. doi: 10.1098/rsob.170207

Figure 6.

Figure 6.

ACKR3 gene silencing inhibits aVP-mediated Gαq signalling, whereas CXCR4 gene silencing does not affect ACKR3 : AVPR1A heteromerization and aVP-mediated Gαq signalling. (a) Representative PLA images for the detection of CXCR4 and receptor–receptor interactions in hVSMC after incubation with NT or CXCR4 siRNA. Ctrl: omission of one primary antibody. Images show merged PLA/4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) signals acquired from z-stack images (n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm. (b) Quantification of PLA signals per cell as in (a). n = 4 independent experiments with n = 10 images per condition and experiment. *p < 0.05 versus cells incubated with NT-siRNA. (c) IP3 production of hVSMC incubated with NT, ACKR3 or CXCR4 siRNA upon stimulation with vehicle (−) or 1 µm aVP (+) for 5 min. n = 4 independent experiments. *p < 0.05 versus vehicle.