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. 2018 Jan 31;8(1):170207. doi: 10.1098/rsob.170207

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© 2018 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — ACKR3-derived TM peptide analogues disrupt ACKR3 : AVPR1A and ACKR3 : CXCR4 heteromeric complexes. hVSMCs were incubated with vehicle, TM2, TM4 or TM7 (10 µM, 30 min at 37°C), washed and fixed for PLA. Typical PLA images for the detection of individual receptors or receptor–receptor complexes are shown. Ctrl: omission of one primary antibody. Images show merged PLA/4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) signals acquired from z-stack images (n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm.