Skip to main content
. 2018 Jan 31;8(1):170207. doi: 10.1098/rsob.170207

Figure 8.

Figure 8.

ACKR3-derived TM peptide analogues interfere with ACKR3 : AVPR1A and ACKR3 : CXCR4 heteromerization and inhibit aVP-induced Gαq signalling in hVSMC. (a,b) Quantification of PLA signals per cell as in figure 7 for individual receptors (a) and receptor–receptor interactions (b). n = 4 independent experiments with n = 10 images per condition and experiment. *p < 0.05 versus cells incubated with vehicle. (c) IP3 production was measured in hVSMC pretreated with vehicle or TM2/4/7 peptide analogues (10 µM, 30 min at 37°C) and then stimulated with either vehicle (−) or aVP (1 µM, 5 min) (+). n = 4 independent experiments. *p < 0.05 versus vehicle-treated cells. (d) β-arrestin 2 recruitment assay (PRESTO-Tango) for AVPR1A. HTLA cells expressing FLAG-AVPR1A-Tango were incubated with either vehicle or ACKR3 TM2/4/7 (10 µM, 30 min at 37°C) and then stimulated with aVP overnight. RLU (%): relative luminescence units in % RLU after treatment with 10 µM aVP (=100%). n = 3 independent experiments.