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Activities of StXyn30A mutants. A 1% soluble beechwood solution in 50 mM acetate buffer (pH 6.0) was incubated with StXyn30A (closed circles) and the R296A (closed squares), R296E (open circles), R296K (open triangles), and R296Q (closed triangles) mutants at 37°C. Hydrolytic activity was determined by the amounts of reducing sugars according to the Somogyi-Nelson method (31).
