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Substrate specificity toward polysaccharidesa
The reactions were performed with 50 mM acetate buffer (pH 6.0) containing 0.5% (wt/vol) substrate, 0.1% (wt/vol) BSA, and 1 μM enzyme at 37°C for 16 h. The reaction was stopped by heating the solutions at 100°C for 20 min. The hydrolytic activity was determined by the amounts of reducing sugars by the Somogyi-Nelson method (31). The assay was performed by using samples in triplicate.
