TABLE 2.

Plasmids constructed and used in this work^a

Bacterial strain	Plasmid	Purpose
E. coli S17-1
E. coli JM109	psb4K5-mRFP	Regulator induction test control
E. coli JM109	psb4K5-AlkR-mRFP	Regulator induction test
E. coli JM109	pBBR1-CYPL-tfu-eGFP	Evaluation of transposon edge
E. coli JM109	pBBR1-CYPLR	Operon functionality
E. coli JM109	pBBR1-Tn::CYPLR	Operon functionality control
E. coli JM109	pBBR1-AlkR	Operon functionality control
E. coli JM109	pBBR1-rha-operon	Operon functionality
E. coli JM109	psb3C5-rha-weak-alkL	Alkane transporter selection and titration
E. coli JM109	psb3C5-rha-weak-ompH	Alkane transporter selection and titration
E. coli JM109	psb3C5-rha-weak-ompH-0644	Alkane transporter selection and titration
P. putida mt-2	pBBR1-CYPLR	Operon functionality
P. putida mt-2	pBBR1-Tn::CYPLR	Operon functionality control
P. putida mt-2	pBBR1-AlkR	Operon functionality control
P. putida mt-2	pBBR1-alkR-operon	Operon functionality control

^aAll the listed plasmids were generated for this study.