Figure 3.

Representative flow cytometry plot for CD25 and FoxP3 expression by regulatory T (Treg) cells. This figure shows the representative flow cytometry plot of CD4⁺ Treg cells expressing CD25 and FoxP3 from a patient with active tuberculosis (TB). The numbers in the quadrants indicate the percentages of cells expressing CD25 and/or FoxP3 among the CD4⁺ population. Q1, CD3⁺ CD4⁺ CD25⁺ FoxP3⁻ (CD4⁺ FoxP3⁻ Treg cells); Q2, CD3⁺ CD4⁺ CD25⁺ FoxP3⁺ (CD4⁺ Treg cells); Q3, CD3⁺ CD4⁺ CD25⁻ FoxP3⁺ (CD4⁺ FoxP3⁺ Treg cells); Q4, CD3⁺ CD4⁺ CD25⁻ FoxP3⁻ T‐cell subset. The same gating strategy was followed for all antigens. [Colour figure can be viewed at wileyonlinelibrary.com]