Figure 5. Effect of MF treatment on Akt-phosphorylation and cell growth in CHIKV infected cells.

( A) The cell lysates of hPDF cells infected with CHIKV181/25 were analyzed for native Akt, p-Akt, and CHIKV antigen levels by western blot analysis. Reduction in levels of CHIKV antigen was found to be concomitant with that of the reduction in the levels of p-Akt. M= ladder, C= Control uninfected cells, I = infected, and DMSO = dimethyl sulfoxide. ( B) Cells were treated with corresponding doses of MF for 4–5 h and infected with CHIKV181/25 (MOI=1). After 72 h pi, virus and drug were removed and cells were washed, replenished with fresh media and incubated for 4 days. ( C) Cells were infected with CHIKV181/25 (MOI=1) and treated with MF at the indicated time points post-infection. After 72 h pi, virus and drug were removed and cells were washed and replenished with fresh medium and incubated for 4 days. B and C: Cells were fixed and stained overnight with 10% neutral buffered formalin containing 0.1% crystal violet and subsequently washed gently under running tap water and observed under the microscope. Data is representative of two repeat experiments.