Table 1.
Characterization of the selected antibodies and their expression in granular cell tumors from various sites
| Marker | Dilution | Supplier | Character |
|---|---|---|---|
| S-100 protein (rabbit polyclonal antibody) | 1:300 | DakoCytomation | E-F hand family (granule cells, glia cells, Schwann cells) |
| Vimentin (mouse monoclonal antibody) | 1:300 | DakoCytomation | Intermediary filament (granule cells) |
| PGP 9.5 (mouse monoclonal antibody) | 1:300 | DakoCytomation | Nerve cells (cell body, axon, granule cells) |
| NSE (mouse monoclonal antibody) | 1:1000 | DakoCytomation | Nerve cells (axis-cylinder process) |
| Calretinin (rabbit polyclonal antibody) | 1:10 | Spring Biosciences | E-F hand family (Schwann cells, central nerve neurons, mast cells) |
| GFAP (mouse monoclonal antibody) | 1:1000 | Novocastra Laboratories | Glia fiber-related acid protein, Schwann cells |
| CD68 (mouse monoclonal antibody)) | 1:300 | Thermo Fisher Scientific | Highly glycosylated membrane proteins, glycoproteins |
| Ki-67 (mouse monoclonal antibody) | 1:400 | DakoCytomation | Proliferation marker |
Abbreviations: NSE neuron-specific enolase, GFAP glial fibrillary acidic protein, PGP protein gene product