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. 2017 Nov 22;21(1):73–83. doi: 10.1093/ijnp/pyx106

Figure 1.

Figure 1.

Timeline of the experiments. Four sets of rats were used in this study, and the figure indicates the experiments performed in each set of rats. In the first week, rats in each set were individually housed during recordings. They were habituated (30 minutes) to the test cage on day 1, to evaluate basal calling, and received vehicle (10:90% dimethyl sulfoxide [DMSO]/polyethylene glycol [PEG]) on day 2. Afterwards, rats received pharmacological treatments and were housed either individually or in pairs during recording, as outlined in the figure. Ultrasonic vocalization (USV) recordings were performed on each testing day and lasted for 2 hours in rats individually housed and 15 minutes in rats housed in pairs. A, amphetamine; C, corticosterone; H, habituation to the test-cage; ME, metyrapone; MI, mifepristone; SP, spironolactone; V, vehicle. The numbers indicate the doses of each drug (in mg/kg). The effects of metyrapone, mifepristone, and spironolactone on calling initiation were evaluated in experiment 2b and 3b, before the administration of amphetamine.