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OMV from Bvg+ and Bvg− strains have distinct protein composition but similar size and total protein amount per vesicle. A, SDS-PAGE and Coomassie blue staining of 10 μg (protein content) of OMV samples from B. pertussis BP536 Bvg+ (lane 1) and its Bvg− derivative BP537 (lane 2). B, Nanoparticle tracking analysis measurement of OMV preparation (1 mg protein/ml) showing the sizes and total number of OMV per ml.
