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. 2017 Dec 7;17(2):233–254. doi: 10.1074/mcp.RA117.000050

Fig. 5.

Fig. 5.

PI4KIIβ controls production of CCL5/RANTES in J774 cells. (A) Expression of genes encoding PI4KIIα and PI4KIIβ in unstimulated cells. (B, C) Expression of PI4KIIα (B) and PI4KIIβ (C) in cells exposed to scrambled siRNA or siRNA targeting PI4KIIβ. (D–G) Expression and production of TNFα (D, E) and CCL5/RANTES (F, G) in cells transfected with scrambled siRNA (scr) or siRNA targeting PI4KIIβ. Expression of genes was analyzed with qPCR (D, F) while concentration of TNFα (E) and CCL5/RANTES (G) in culture supernatants was measured with ELISA. NS, unstimulated cells, LPS, cells stimulated with 100 ng/ml LPS for 4 h. Data are mean ± s.d. from three experiments run in duplicates (A–D, F) or in triplicates (E, G). ***, Significantly different at p < 0.001 from cells transfected with scrambled siRNA and left unstimulated (C) or stimulated with LPS (F, G).