Fig. 3.

Effect of environmental factors on expression of β-amylases analyzed by qRT-PCR. (A) Relative mRNA expression levels of BA1–4 in T. vaginalis cultivated in standard TYM medium. Expression levels of β-amylases were normalized using the DNATopII reference gene. (B) Relative mRNA expression level of BA1–4 in T. vaginalis cultivated in TYM when maltose is replaced with glycogen or in standard TYM medium with a restricted level of iron. Data are expressed as -fold increases relative to the expression of the corresponding gene in standard TYM medium. Error bars represent the standard deviation of the mean.