Table 2.
Primers used for the deletion/confirmation of deletion by PCR of A46R, A52R, K7R, B15R, B8R, B19R and B6R-B10R open reading frames (ORFs). Restriction enzymes cleavage sites are underlined.
| Locus | Sequence of Primers (5′→3′) | Size of Amplified Product (bp) | |
|---|---|---|---|
| Parental Virus | Deletion Mutant | ||
| A46R | LFA46R-Aat: CACGATGACGTCAGAGGAGTTAT RFA46R-Bam: ATTTAAGGATCCAGAACGGCAAC |
1342 | 774 |
| A52R | LF′A52R-Eco: ATTAGAGAATTCTACGATTAACGA RFA52R-Bam: TCTGCCGGATCCAATGTAGTAATG |
1320 | 747 |
| K7R | K7R-F: TATGATCATGTGAGAATACTAAAATTCC K7R-R: CCGAATTGGGTAGACGATGTATGAATCC |
1048 | 626 |
| B15R | LF′B15R-Aat: TTCTTTGACGTCTGTTTTCCTGAAG RFB15R-Bam: GTGTCGGGATCCGAATTAGCATATT |
1128 | 701 |
| B8R | LFB8R-AatII-F: TTTTTTGACGTCATTGACTCGTCTACTATTC RFB8R-BamHI-R: TTTTTTGGATCCAAACAGCGGACACATTGC |
1519 | 706 |
| B19R | LFB19R-AatII-F: TTTTTTGACGTCGAGAAAGTTAAGAAGATAC RFB19R-BamHI-R: TTTTTTGGATCCAGTTCTATCATAATCATC |
1782 | 726 |
| B6R-B10R | LFB6R-AatII-F: GGAATGACGTCCTCCCAATATGTG RFB10R-BamHI-R: CGGGATCCAGTAGATATGATCTATATTC |
3283 | 725 |