Figure 6.

Accumulation of phosphorylated nuclear lamins at INM-budding sites of HCMV capsids during nuclear egress. HCMV-infected (a–d) or uninfected (mock; (e)) HFFs were stained with phosphorylation-independent (a,b) or phosphorylation-dependent (c–e) lamin A/C primary antibodies and gold-tagged secondary antibodies. Samples were analysed by TEM, 35,970-fold magnification. NE, nuclear envelope; open arrowheads, intranuclear HCMV capsids; filled arrowheads, HCMV capsids budding at nuclear membranes.