Figure 5.

ZIKV infected multiple areas of CNS. (A) Horizontal section of the brain of the ZIKV-infected mouse was stained with anti-E protein. The area I and II was amplified to show the infection of ZIKV (brown color stand for the positive infection; (B–D) Immunohistochemistry (IHC) analysis of CNS tissues after being infected with PRVABC59. The 1-day-old C57BL/6 mice were infected i.p. with 10⁶ TCID₅₀ PRVABC59 per mouse or PBS. Mice were sacrificed at 11 dpi to isolate brain tissue for sectioning and staining by the IHC method. Representative pictures from PRVABC59-infected brain tissue slides were selected to show (B) brain cortex; (C) spine; (D) hippocampus; (E) the same as in (B–D) but stained with IFA for hippocampus, olfactory bulb, cerebrum, and cortex. Scale bars: A left 500 μm, A middle, 100 μm, A right, 20 μm; B 50 μm; C 100 μm; D 100 μm.