Figure 6.

Replicated ZIKV levels in brain and spinal cord of the PRVABC59-infected neonatal C57BL/6 mice. The 1-day-old mice were infected i.p. with 10⁶ TCID₅₀ PRVABC59 per mouse for different days as indicated. The mice were then dissected after euthanasia at 0, 2, 4, 6, 8, 10 and 12 dpi to isolate the organs and tissues as indicated: brain and spinal cord. Real time RT-PCR was performed to determine the ZIKV RNA level in the isolated or collected organs and tissues. For each length of time kept, we used three mice. The average number of the RNA copies from the real time RT-PCR was obtained statistically with GraphPad Prism Software version 5.01. (The statistical significance was not determined. The graph only showed the means ± S.D.).