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Construction of recombinant MVA vector by homologous recombination. A plasmid that contains the gene or transgene of interest is constructed and used to transfect an MVA-infected cell. TK- Recombinant MVA vectors are produced by homologous recombination; TK: thymidine kinase gene; M: marker gene; TG: foreign gene; P: promoter; O: Origin of plasmid replication; N: Marker gene for plasmid selection.
