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. 2018 Jan 10;15(3):2830–2836. doi: 10.3892/etm.2018.5731

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of biochanin A on the migration of A427 cells imparted during coculture. Human lung cancer A427 cell lines were treated with or without 40 µM of biochanin A for 24 h and cocultured with AML-193 cells at a ratio of 1:15 for 8 h. CM was used for the healing of wounded monolayers of A427 cells for 48 h. Drug treatment showed notable inhibition of wound healing, compared with the control. (A) Images demonstrate the initial wounds of A427 cells and after 48 h in the NTCM and TCM groups. (B) Quantification of three randomly selected scratched areas. Data are represented as mean + standard deviation of three individual experiments. *P<0.05 vs. NTCM. CM, conditioned medium; NTCM, non-treated conditioned medium; TCM, treated conditioned medium.