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. 2018 Jan 4;15(3):2533–2540. doi: 10.3892/etm.2018.5689

Figure 3.

Figure 3.

SI of spleen cells. One-way analysis of variance was used for statistical analysis, while pairwise comparison was performed among A1 to C2 groups. SI (A1, A2, B1, B2, C1 and C2): F=74.675, P<0.05. A1, 2× intramuscular immunization with pcDNA3.1 (100 µg); A2, 2× intramuscular immunization with pcDNA/Gpd-IL-2 (100 µg); B1, 1× intramuscular immunization with pcDNA/Gpd-IL-2 (100 µg) + 1× nasal immunization with pcDNA/Gpd-IL-2 (100 µg); B2, 1× intramuscular immunization with pcDNA/Gpd-IL-2 (100 µg) + 1× nasal immunization with pcDNA/Gpd-IL-2 (100 µg) and CpG-ODN (10 µg); C1, 1× intramuscular immunization with pcDNA/Gpd-IL-2 (100 µg) + 1× nasal immunization with Gpd-IL-2 recombinant protein (100 µg); C2, 1× intramuscular immunization with pcDNA/Gpd-IL-2 (100 µg) + 1× nasal immunization with Gpd-IL-2 recombinant protein (100 µg) and CpG-ODN (10 µg); SI, stimulation index. *P<0.05 vs. A2/B1/B2/C1/C2; **P<0.05 vs. B1; ***P<0.05 vs. B2/C1/C2; #P<0.05 vs. C2.