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Detection of apoptosis in rat prostate tissue after 28 days of treatment in the (A) testosterone, (B) 3-MA, (C) control and (D) rapamycin groups. 4′,6-diamidino-2-phenylindole dye staining was used on the nucleus. When this dye is excited it emits blue light. The fluorescent dye was combined with apoptotic cells and a fluorescence microscope was used to observe the green fluorescence under the excitation of the blue light. Scale bar, 100 mm. Magnification, ×40. 3-MA, 3-methyladenine.
