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. 2017 Oct 17;11(1):1–10. doi: 10.1007/s12195-017-0511-x

Figure 1.

Figure 1

Girdin regulates migration through 3D isotropic bulk collagen matrices and microtracks. (a) Western blot confirming the knockdown of Girdin in siRNA-treated MDA-MB-231 cells; (b) Time-lapse phase contrast images of control (scrambled) siRNA-treated (top), and Girdin siRNA-treated (bottom) MDA-MB-231 cells showing migration distance travelled by siRNA-treated cells in a 100 min time interval. Yellow dashed outlines indicate the initial positions of cells; c Rose plots showing trajectories of control and Girdin siRNA-treated cells in 3D collagen matrices, # of cells presented = 15; (d) Phase contrast (left) and confocal reflectance (right) images of a control siRNA-treated MDA-MB-231 cell (double arrowheaded) migrating through a 3D in vitro microfabricated collagen microtracks, (e) Average travelled distance of control and Girdin siRNA-treated MDA-MB-231 cells in microtracks over a period of more than 6 h. (f) Comparison of average migration speeds and (g) directional persistence of control and Girdin siRNA-treated MDA-MB-231 cells in 3D collagen isotropic matrices and 3D collagen microtracks. # cells per treatment = 32–45, scale bars 25 µm, *p value < 0.05, **p value < 0.01.