Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 1;31(23-24):2325–2330. doi: 10.1101/gad.308254.117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Reginato et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 3. — RPA bound to ssDNA directs the nuclease cleavage of adjacent dsDNA by the MRX–Sae2 complex. (A) Representative nuclease assays showing the degradation of 3′ tailed (PC216 and PC216_C_50 nt), 5′ tailed (oligonucleotides PC216_50 nt and PC217), and blunt-ended DNA (PC216 and PC217) by MRX–Sae2 without or with RPA. The red asterisk indicates the location of the ³²P label. (B) Representative nuclease assays as in A showing the degradation of tailed (oligonucleotides PC204 and PC217) and blunt-ended (PC216 and PC217) DNA by MRX–Sae2 with or without RPA.