Skip to main content
. 2017 Dec 1;31(23-24):2343–2360. doi: 10.1101/gad.302646.117

Figure 5.

Figure 5.

ARID5B coordinately regulates the expression of TAL1 targets in T-ALL cells. (A) DNA-binding motifs significantly enriched in regions bound by ARID5B in Jurkat cells are shown with P-values. (B) Co-occupancy by TAL1, GATA3, RUNX1, and MYB at the ARID5B-bound gene loci (±1 kb from the binding sites) in Jurkat cells was analyzed by ChIP-seq. (C) A GSEA plot showing the overall pattern of gene expression changes of TAL1 target genes upon ARID5B knockdown (KD) in Jurkat cells. Each solid bar represents one gene within the gene set. (D) ChIP-seq gene tracks show the binding of ARID5B, TAL1, GATA3, RUNX1, and RNAPII and the presence of H3K27ac at the ALDH1A2 promoter in Jurkat cells. See the legend for Figure 1F for details. (E) mRNA expression of ALDH1A2 in Jurkat cells on day 3 after ARID5B knockdown was measured by qRT–PCR. The data represent the mean ± SD of duplicate samples. (***) P < 0.001 by two-sample two-tailed t-test. (F) Luciferase activity driven by the ALDH1A2 promoter was measured after ARID5B knockdown in Jurkat cells. The luciferase activity on days 3 and 5 was normalized to shGFP (control). The data represent the mean ± SD of duplicate samples. (*) P < 0.05, (***) P < 0.001 by two-sample two-tailed t-test.