Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 1;31(23-24):2343–2360. doi: 10.1101/gad.302646.117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Leong et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 6. — ARID5B positively regulates the expression of the TAL1 complex in T-ALL cells. (A–D) ChIP-seq gene tracks showing the binding of ARID5B, TAL1, and RNAPII as well as the H3K27ac signal at the enhancer/promoter regions of TAL1 (A), GATA3 (B), RUNX1 (C), and MYB (D) in Jurkat cells. See the legend for Figure 1F for details. (E) mRNA expression of ARID5B, TAL1, GATA3, RUNX1, and MYB in Jurkat cells on day 3 after ARID5B knockdown was measured by qRT–PCR. The relative gene expression was normalized to the ERCC spike-in exogenous control (E130). The data represent the mean ± SD of duplicate samples. (***) P < 0.001 by two-sample two-tailed t-test. (F) Western blot analysis for protein expression of ARID5B, TAL1, GATA3, RUNX1, MYB, and α-tubulin (loading control) in Jurkat cells on day 3 after infection with lentivirus expressing shRNA.