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. 2017 Dec 1;31(23-24):2376–2390. doi: 10.1101/gad.307637.117

Figure 4.

Figure 4.

Afadin regulates Rab8-mediated apical docking during de novo lumen formation. (A–N) Paraffin sections of E15.5 and E12.5 wild-type and AfapancKO pancreata immunostained for lumenal Muc1; TJ protein ZO1; vesicular GTPases Rab11, Rab8, and Cdc42; and E-cadherin (Ecad; epithelial membranes). Insets in A, A′, C, C′, E, E′, G, and G′ are shown at higher magnification in B, B′, D, D′, F, F′, H, and H′, respectively. Insets in M and N show Cdc42 and Muc1 staining, excluding E-cadherin. Bars, 10 μm. (A–D′) Representative images from five independent experiments are shown. n = 5 embryos per genotype. (E–H′) Representative images from four independent experiments are shown. n = 4 embryos per genotype. (I–N) Representative images from three independent experiments are shown. n = 3 embryos per genotype. (O–V) Pancreatic sphere assays seeded with cells from E10.5–E11.5 wild type and AfapancKO. After 5 d (O–P′) or 1 d (Q–V) in culture, spheres were immunostained. Representative images from four independent experiments are shown. n = 4 embryos per genotype. Bars: O–P′, 10 µm; Q–V, 5 µm. See also Supplemental Figure S4 and Supplemental Movies S4 and S5.