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. 2017 Dec 1;31(23-24):2416–2429. doi: 10.1101/gad.308163.117

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© 2018 Bao et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — The requirement for Prp2 activity can be bypassed in the absence of Snu17. Pre-mRNA splicing in extracts from prp2-1 or prp2-1 Δsnu17 yeast cells without heat inactivation of Prp2 (A) or after heat inactivation of Prp2 at 35°C (B). Splicing was performed without or after addition of affinity-purified Snu17/Bud13 (100 or 200 ng as indicated). RNA was separated by denaturing PAGE and visualized using a PhosphorImager. The identities of the ³²P-labeled RNA species are indicated at the right. The percentage of mRNA formed after 30 min is indicated below.