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. 2018 Feb 1;18:46. doi: 10.1186/s12906-018-2086-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — The composition of PF-GPD was analyzed by SHIMADZU API 4000 LC/MS/MS system equiped with SHIMADZU VP-ODS column (4. 6 mm × 150 mm). The mobile phase A was 1% formic acid, mobile phase B was acetonitrile, the gradient elute condition was 0–5 min 45% mobile phase B, flow velocity is 0.5 mL/min, and the column temperature was 40 °C. The total ion chromatography of PF-GPD and extract ion chromatography of the L-leucine, L-lysine and L-valine were shown in picture a, b, c and d, respectively