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Overexpression of mSp5 inhibited the growth of HCT116 cells. (A) Lysates from HCT116 cells transfected with FLAG-tagged mSp5 were subjected to immunoblotting analysis with an anti-FLAG antibody. Tubulin was used as a loading control. (B) Growth of HCT116 cells transfected with mock or mSp5 expression vectors was measured by CellTiter-Glo assay. Results are shown as mean ± standard deviation of 3 wells. *P=0.009, Student's t-test. mSp5, mouse specificity protein 5.
