Figure 4.

Knockdown of p27 partially restores the growth of HCT116 cells overexpressing mSp5. (A) Reverse transcription-quantitative polymerase chain reaction analysis of p27 expression in HCT116 cells transfected with the indicated expression constructs (*P=0.028,**P=0.007, Student's t-test). (B) Cell proliferation in HCT116 cells transfected with the indicated constructs. Growth was quantified by CellTiter-Glo assay. Results are expressed as the means ± standard deviation of three independent experiments (*P=0.04, **P=0.03, Student's t-test. mSp5, mouse specificity protein 5; si, small interfering RNA.