Figure 3.

mRNA expression of CXCR3, CXCL9 and CXCL10 on T cells isolated from CRC tumor tissues. T cells were purified from post-digestion cell suspension of tumor tissue of colorectal cancer using anti-CD3 microbeads. The eluted cells were subject to lysis and purification of mRNA using a Qiagen RNA purification column. Quantitative polymerase chain reaction analysis was performed to determine the levels of RNA of each transcription factor, which represents each subset of immune cell. Data are presented as a heat map to associate the level of mRNA with the intensity of infiltration. CXCR3, CXC motif chemokine receptor 3; CXCL, CXC motif chemokine ligand.