Figure 1.

Effects of different concentrations of BJOE on cell viability of ECA109 cells determined by an MTT assay. ECA109 cells were inoculated in 96-well plates at a density of 5×10³ cells/ml. The cells were cultured in an incubator containing 5% CO₂ at 37°C. The medium was removed and replaced with fresh medium supplemented with different BJOE concentrations (1.25, 2.5, 5, 10, 20, 40, 80, and 100 mg/ml), when the monolayers had reached confluency. An untreated cell culture medium was used as an experimental control. After 24 and 48 h, cell viability was determined. BJOE inhibited the growth of ECA109 in a concentration- and time-dependent manner. The treatment with 2.5 mg/ml BJOE for 24 h did not significantly affect the optical density of ECA109 cells. Data are shown as mean ± standard deviation. *P<0.05 and **P<0.01, compared with the control; NS, no significance, compared with the control; BJOE, Brucea javanica oil emulsion.