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. 2017 Dec 21;19(1):10. doi: 10.3390/ijms19010010

Figure 5.

Figure 5

Role ERK1/2 in PA-regulated MMP-2 and TIMP-2 expression in HeLa cells. (A) Cells were treated with various concentrations of PA (0 to 30 μM) for 24 h, then harvested and lysed for measurement target proteins by western blotting; (B) cells were treated with or without PA in the absence or presence of PD98059 (specific MEK1/2 inhibitor) for 24 h, followed by measurement of migration and invasion; (C) cells were treated as above for 24 h, followed by measurement of relative wound width; (D,E) Protein and mRNA expression of MMP-2 and TIMP-2 were measured by western blotting assay and RT-qPCR. Values are means and standard errors of 3 replicates. ** p < 0.01 versus control; # p < 0.01 versus only PA treatment. Scale bar, 50 μm.