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. 2017 Dec 21;19(1):10. doi: 10.3390/ijms19010010

Figure 6.

Figure 6

Effect of PA on TPA-induced ERK1/2 activation, MMP-2 expression, and migration/invasion of HeLa cells. (A) Cells were pretreated with or without PA (20 μM) for 2 h, then added TPA (50 ng/mL) for different times (0, 30, 60, 90 and 120 min), then harvested and lysed at the indicated times for measurement of target proteins by western blotting; (B) cells were pretreated with PA (20 to 30 μM) for 2 h, then in the absence or presence of TPA (50 ng/mL) for 24 h, followed by measurement of migration and invasion; (C) cells were treated as above for 24 h, followed by measurement of relative wound width; (D,E) cells were pretreated with PA (20 to 30 μM) for 2 h, then in the presence or absence of TPA (50 ng/mL) for 24 h, followed by measurement of ERK1/2 phosphorylation and MMP-2 protein and mRNA levels by western blotting and RT-qPCR assay. Values are means and standard errors of 3 replicates. ** p < 0.01 versus control; # p < 0.01 versus only TPA treatment. Scale bar, 50 μm.