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. 2018 Jan 2;19(1):14. doi: 10.3390/ijms19010014

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antibodies against MOR, DOR1 and DOR2 recognize endogenous receptors in zebrafish larval sections by immunohistochemistry. Lateral (a, b, c, d) and dorsal (e, f, g, h) views of opioid receptors protein distribution in head and spinal cord sections from zebrafish larvae 5 days post fertilization. Sections were obtained as described in the Materials and Methods section and subjected to immunohistochemistry with rabbit IgGs (Control), MOR, DOR1 or DOR2 antibodies. Abbreviations: hypothalamus (H), hindbrain (Hb), inferior lobullum (IL), interpeduncular nucleus (IN), medulla oblongata (MO), optic tectum (OT), pallium (P), semicircular canal (SC) and telencephalon (T). A representative experiment is shown (n = 3). Scale bar: 100 µm. A representative experiment is shown (n = 3).