Figure A1.

0.8% agarose gel electrophoresis of a total of 35 viable clones screened by 3′-HA PCR. 500 ng gDNA templates (numbered as 1 to 35) were amplified at the annealing temperature of 65 °C for 30 cycles, with gDNA template from untransfected (Untft) E14-Bra-GFP mESCs cultured on gelatine as a negative control. A non-template control (NTC) was also included where template gDNA was substituted by nuclease-free water. Eighteen out of 35 viable clones show bright positive bands and three show weaker bands (indicated by asterisks) of the correct size of 3380 bp. L, 1 kb ladder.