Table 3.

Inter-day and intra-day assay precision (% RSD) and accuracy (% Bias) values for the HPLC–UV method for the quantification of 5-FU and 5-FDHU in rat plasma.

Nominal Con. (µg/mL)	Intra-Day (n = 6)			Inter-Day (n = 6)
	Observed Con. (µg/mL)	Accuracy Bias (%)	Precision RSD (%)	Observed Con. (µg/mL)	Accuracy Bias (%)	Precision RSD (%)
5-FU
0.1	0.111 ± 0.01	10.6	6.58	0.102 ± 0.01	2.02	11.5
0.5	0.528 ± 0.04	5.69	1.03	0.487 ± 0.04	−2.69	7.35
1	0.971 ± 0.03	−2.92	2.70	0.966 ± 0.03	−3.45	3.32
5	5.018 ± 0.05	0.37	1.08	4.965 ± 0.12	−0.70	2.43
10	10.02 ± 0.12	0.21	1.16	10.04 ± 0.12	0.40	1.17
50	50.15 ± 0.13	0.30	0.26	49.98 ± 0.02	−0.04	0.05
5-FDHU
0.1	0.101 ± 0.01	0.60	8.88	0.092 ± 0.01	−7.98	5.70
0.5	0.489 ± 0.04	−2.23	7.43	0.503 ± 0.01	0.51	1.74
1	1.044 ± 0.04	4.41	3.44	1.025 ± 0.04	2.45	3.61
5	4.766 ± 0.25	−4.68	5.20	4.867 ± 0.17	−2.65	3.50
10	10.26 ± 0.31	2.61	3.03	10.11 ± 0.19	1.05	1.84
50	50.16 ± 0.30	0.31	0.59	49.99 ± 0.04	−0.02	0.09

Data are expressed as the means ± SD. Precision (%RSD) = SD/C_obs × 100. Accuracy (%Bias) = (C_observation − C_nominal)/C_nominal × 100.