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. 2017 Dec 23;19(1):38. doi: 10.3390/ijms19010038

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Reactivity of rat anti-rNS1 immune serum against ZIKV NS1 protein. RIPA lysates obtained from Vero cells infected with ZIKV strains MR766, BR15, and PF-25013-18 (PF13) or live-attenuated YFV 17D vaccine (YF-17D) or mock-infected (m.i.) were incubated with anti-NS1^1-151 (a) or anti-rNS1; (b) immune serum (dilution 1:500 and 1:1000 respectively) by immunoblot assay. The samples were heat denatured (+) or not (−) before immunoblot analysis. To the right of the blot, the open head arrow indicates the position of the NS1 monomer, and the black head arrow indicates the position of the NS1 dimer. The molecular weight (MW) of protein size markers is indicated to the left of the blot.

Reactivity of rat anti-rNS1 immune serum against ZIKV NS1 protein. RIPA lysates obtained from Vero cells infected with ZIKV strains MR766, BR15, and PF-25013-18 (PF13) or live-attenuated YFV 17D vaccine (YF-17D) or mock-infected (m.i.) were incubated with anti-NS1^1-151 (a) or anti-rNS1; (b) immune serum (dilution 1:500 and 1:1000 respectively) by immunoblot assay. The samples were heat denatured (+) or not (−) before immunoblot analysis. To the right of the blot, the open head arrow indicates the position of the NS1 monomer, and the black head arrow indicates the position of the NS1 dimer. The molecular weight (MW) of protein size markers is indicated to the left of the blot.