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Reactivity of anti-rNS1 immune serum against ZIKV NS1 synthesized in human cells. A549 cells were infected 48 h with ZIKV strains MR766 and BR15 or mock-infected and analyzed by IF assay. Fixed cells were permeabilized with non-ionic detergent and then incubated with rat anti-rNS1 immune serum at dilution 1:2000. Magnification: ×400.
