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. 2017 Dec 25;19(1):53. doi: 10.3390/ijms19010053

Figure 4.

Figure 4

Millimolar minoxidil inhibits HIF prolyl hydroxylase. (A) (Left) A VHL capture assay was performed in the presence of minoxidil (MXD) at various concentrations, and the resultant blots were probed with the antibody to Flag (VHL). (Right) The same assay was repeated utilizing a chemically hydroxylated peptide and MXD (5 mM) (n = 5), * p < 0.05 versus untreated group; (B) HaCaT cells were treated for 4 h with MXD or MG132 (10 μM), a proteasome inhibitor, and were lysed to obtain nuclear extracts. Blots were probed with an anti-HIF-1α antibody and an anti-hydroxylated HIF-1α antibody (n = 5). * p < 0.05 versus untreated group. Western blot results are representative of five independent experiments. TBP: TATA binding protein.