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. 2017 Dec 26;19(1):63. doi: 10.3390/ijms19010063

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mature MDDCs promote the differentiation of naïve T-cells. Naïve T-cells obtained from HC, SA and NSTEMI (5 patients for each group) were co-cultured for 6 days with autologous mMDDCs at 10:1 ratio. Afterwards, T-cell differentiation was analyzed by flow-cytometry as described in Figure S2. (A) NSTEMI patients showed increased Th1 differentiation compared to HC and SA (p for trend = 0.006). No differences in absolute Treg frequency were observed among the three groups of study. (B) Histograms show the ratio between the percentages of differentiated Th1/Treg cells. NSTEMI and SA patients have significantly higher ratio favoring Th1 cells compared to HC, with NSTEMI having the higher ratio (p for trend = 0.014). Data are expressed as means ± SEM.