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. 2017 Dec 26;19(1):63. doi: 10.3390/ijms19010063

Figure 4.

Figure 4

Naïve T-cell differentiation after T-cell receptor (TCR) activation and exposure to cytokine mixture. Naïve CD4+ T-cells were stimulated for six days with anti-CD3/-CD28-coated beads alone (blue histograms) or in presence of a cytokine mixture (red histograms) including IL-2, IL-12, IL-1β, IL-6, TGF-β, IL-23, IL-10, anti-human-IL-4. The cytokine cocktail reduced the frequency of Treg as assessed by flow-cytometry (panel (A)) and the expression of Foxp3 lineage specific gene (panel (B)) and increased the frequency of Th1 and Th17, as assessed by flow-cytometry (panel (C,E)) and the expression of their lineage specific genes T-bet and Rorγ-t, as assessed by RT-qPCR (panel (D,F)). No statistical differences were observed among the three groups of study under these experimental conditions. Cumulative data from 10 NSTEMI10 SA and 10 HC are expressed as mean ± SEM. Th1, Th17 and Treg characterization by flow-cytometry has been described in Figure S2.