Table 1.
Fold changes of microRNAs (miRNAs) differentially expressed in subcutaneous adipose tissue (SAT) of obese individuals before (O) and after weight loss (PO), as well as of normal-weight subjects (N) assessed by the next generation sequencing (NGS) and real-time PCR methods.
| SAT-O vs. SAT-N | SAT-PO vs. SAT-O | SAT-PO vs. SAT-N | ||||
|---|---|---|---|---|---|---|
| NGS | Real-Time PCR | NGS | Real-Time PCR | NGS | Real-Time PCR | |
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Proadipogenic | ||||||
| hsa-miR-15a-5p * | −2.992 | −2.286 | ||||
| hsa-miR-21-5p * | 2.728 | |||||
| hsa-miR-107 | −3.057 | −3.734 | ||||
| hsa-miR-143-5p * | −2.126 | |||||
| hsa-miR-146b-3p | 7.253 | 2.710 | −4.722 | −2.972 | ||
| hsa-miR-150-5p * | 2.128 | |||||
| hsa-miR-194-5p | −3.029 | −3.645 | −2.109 | −7.00 | ||
| hsa-miR-199b-5p | 2.803 | |||||
| hsa-miR-210 | −2.130 | −2.617 | ||||
| hsa-miR-214-5p * | 2.266 | |||||
| hsa-miR-335-3p | 2.574 | |||||
| hsa-miR-450a-5p | 2.282 | 2.100 | ||||
| Antiadipogenic | ||||||
| hsa-miR-18a-3p | −3.703 | −3.699 | ||||
| hsa-miR-23a-5p | 2.093 | |||||
| hsa-miR-27a-5p * | 2.363 | 2.512 | ||||
| hsa-miR-29a-3p * | 2.010 | |||||
| hsa-miR-130b-3p | −4.053 | −2.787 | ||||
| hsa-miR-196a-5p | 4.031 | 3.162 | 4.047 | 2.97 | ||
| Involved in adipocyte browning | ||||||
| hsa-miR-125b-5p | 2.247 | 2.01 | ||||
| Involved in inflammation and immune response | ||||||
| hsa-miR-15b-3p * | −5.090 | −6.432 | ||||
| hsa-miR-16-2-3p * | −7.972 | −6.263 | ||||
| hsa-miR-20b-5p * | −2.936 | −3.518 | ||||
| hsa-miR-22-3p * | 3.001 | 2.011 | ||||
| hsa-miR-32-5p * | −2.452 | |||||
| hsa-miR-92b-3p * | 2.228 | |||||
| hsa-miR-93-5p | −3.602 | −3.358 | ||||
| hsa-miR-106a-5p * | −3.470 | −4.302 | ||||
| hsa-miR-122-5p * | −5.792 | |||||
| hsa-miR-125a-5p * | 2.150 | 1.96 | ||||
| hsa-miR-125b-1-3p * | 2.063 | |||||
| hsa-miR-142-3p * | −5.215 | |||||
| hsa-miR-142-5p * | −6.087 | −5.233 | ||||
| hsa-miR-146b-5p * | 5.077 | −3.986 | ||||
| hsa-miR-182-5p * | −15.039 | |||||
| hsa-miR-223-3p * | 5.804 | 3.041 | −27.825 | −8.036 | −4.794 | −2.72 |
| hsa-miR-3200-3p | −3.103 | −3.802 | ||||
| Involved in lipid metabolism | ||||||
| hsa-miR-106b-5p * | −3.778 | −3.984 | ||||
| hsa-miR-144-3p * | −11.390 | −14.187 | ||||
| hsa-miR-185-5p | −2.836 | −3.303 | ||||
| Involved in atherosclerosis | ||||||
| hsa-miR-365a-3p | 2.559 | |||||
| hsa-miR-365b-3p | 2.559 | |||||
| Involved in insulin signaling | ||||||
| hsa-miR-96-5p | −22.917 | −9.251 | −13.913 | −15.01 | ||
| hsa-miR-128 | −2.418 | |||||
| Involved in oncogenesis | ||||||
| hsa-let-7e-3p | 2.100 | |||||
| hsa-miR-16-5p | −2.755 | |||||
| hsa-miR-17-3p | −2.066 | |||||
| hsa-miR-18a-5p | −2.410 | −4.324 | ||||
| hsa-miR-18b-5p | −4.240 | −6.464 | ||||
| hsa-miR-25-3p | −2.289 | −2.448 | ||||
| hsa-miR-28-5p | 2.086 | |||||
| hsa-miR-95 | 3.118 | |||||
| hsa-miR-106b-3p | −3.636 | |||||
| hsa-miR-144-5p | −8.083 | −8.337 | ||||
| hsa-miR-183-5p | −14.823 | −3.723 | −20.421 | −7.62 | ||
| hsa-miR-192-5p | −3.234 | −3.408 | ||||
| hsa-miR-193b-5p | 2.500 | 1.97 | ||||
| hsa-miR-205-5p | −10.897 | −5.541 | −10.030 | −10.73 | ||
| hsa-miR-215 | 2.015 | |||||
| hsa-miR-223-5p | 2.894 | −5.798 | −2.003 | |||
| hsa-miR-301b | 2.972 | |||||
| hsa-miR-330-3p | 2.452 | |||||
| hsa-miR-340-3p | 2.073 | |||||
| hsa-miR-361-3p | 2.504 | |||||
| hsa-miR-363-3p | −5.212 | −4.293 | ||||
| hsa-miR-423-5p | −2.430 | |||||
| hsa-miR-424-3p | −2.020 | |||||
| hsa-miR-425-5p | −3.062 | −2.103 | ||||
| hsa-miR-451a | −9.299 | −11.488 | ||||
| hsa-miR-483-3p | 2.681 | 2.860 | ||||
| hsa-miR-489 | 2.440 | |||||
| hsa-miR-501-3p | −3.074 | |||||
| hsa-miR-518b | 2.705 | |||||
| hsa-miR-548d-5p | −3.669 | −3.766 | ||||
| hsa-miR-550a-3p | −3.100 | |||||
| hsa-miR-576-5p | −3.083 | −3.587 | ||||
| hsa-miR-584-5p | −2.741 | −2.377 | ||||
| hsa-miR-615-3p | 3.163 | |||||
| hsa-miR-628-5p | 2.569 | 2.504 | ||||
| hsa-miR-629-5p | −2.241 | −2.261 | ||||
| hsa-miR-652-3p | −2.807 | −2.653 | ||||
| hsa-miR-671-3p | 2.878 | |||||
| hsa-miR-874 | 2.070 | |||||
| hsa-miR-891a | 2.984 | |||||
| hsa-miR-941 | 2.404 | −2.239 | ||||
| hsa-miR-1260b | 2.460 | 1.524 | 4.854 | 2.30 | ||
| hsa-miR-1271-5p | 2.540 | |||||
| hsa-miR-1285-3p | −2.396 | |||||
| hsa-miR-3688-3p | −5.070 | −5.732 | ||||
| hsa-miR-4662a-5p | 3.410 | |||||
| hsa-miR-4732-3p | −3.347 | −3.124 | ||||
| hsa-miR-5683 | 5.557 | |||||
| hsa-miR-6716-3p | 2.481 | |||||
| Involved in ageing | ||||||
| hsa-miR-141-3p | −5.518 | −8.255 | ||||
| hsa-miR-369-5p | 2.554 | |||||
| Involved in cardiovascular diseases | ||||||
| hsa-miR-589-5p | 2.193 | |||||
| hsa-miR-3615 | −5.278 | −4.355 | ||||
| Involved in neurodegenerative disorders | ||||||
| hsa-miR-15b-5p | −2.811 | −2.753 | ||||
| hsa-miR-132-5p | 2.921 | |||||
| hsa-miR-151a-5p | 2.228 | |||||
| hsa-miR-548ay-5p | −3.505 | −3.731 | ||||
| hsa-miR-664a-3p | 2.102 | |||||
| hsa-miR-3607-3p | 2.706 | |||||
| hsa-miR-3909 | 2.865 | |||||
| Unknown function | ||||||
| hsa-miR-450b-5p | 3.284 | 3.723 | 2.605 | 7.13 | ||
| hsa-miR-486-3p | −12.206 | −11.022 | ||||
| hsa-miR-486-5p | −9.535 | −4.464 | −9.118 | −8.83 | ||
* miRNA also associated with other obesity-related pathologies.