Table 3.
Cross-reactivity data of R12.51 ssDNA MRE in river water/buffer mix.
| Target | Normalized Average Fluorescence | Standard Deviation | p-Value | Selective Ratio |
|---|---|---|---|---|
| Fipronil | 3.863 | 0.090 | - | - |
| Fipronil Sulfone | 2.863 | 0.180 | 4.98 × 10−4 | 1.34 |
| Fipronil Sulfide | 4.333 | 0.944 | 2.19 × 10−1 | 0.89 |
| Fipronil Desulfinyl | 2.902 | 0.245 | 15.5 × 10−3 | 1.33 |
| Ethiprole | 3.157 | 0.148 | 1.06 × 10−3 | 1.22 |
| Atrazine | 0.804 | 0.302 | 3.66 × 10−5 | 4.80 |
| Propanil | 0.764 | 0.509 | 2.44 × 10−4 | 5.05 |
| Malathion | 0.725 | 0.355 | 5.98 × 10−5 | 5.32 |
| BSA | 20.41 | 1.118 | 6.95 × 10−6 | 0.19 |
| River water/Buffer Mix (No Targets) | −0.549 | 1.779 | 6.37 × 10−3 | −7.035 |
For each negative target (200 nM), normalized average fluorescence is given with 1× standard deviation. Each set of experiments was performed in triplicate. A one tailed student’s t-test was performed to calculate the p-value between fipronil and cross-binding targets. The selectivity ratio represents the normalized average fluorescence for fipronil divided by the normalized average fluoresce for the cross-binding target, indicating R12.51’s level of preference for fipronil compared to the cross-binding target.