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. 2017 Dec 29;19(1):93. doi: 10.3390/ijms19010093

Figure 3.

Figure 3

Effect of miR27b-SP on hC27bSPs and bC7aSPs liver and intravascular lipids through Oil Red O (ORO) staining. (A–E) Lateral view of the whole-mount ORO staining of representative larvae fed with high-fat diet (HFD) (40× magnification, scale bars: 200 µm). Enlargement at the trunk and posterior level is shown in panels ′ and ″ (110× magnification, scale bars: 100 µm). (F) The percentages of hC27bSPs and bC27bSPs larvae with steatosis and intravascular lipids was calculated through ORO staining. The ORO staining was performed in triplicate with on average 60–80 larvae per groups. The asterisk represents statistically significant differences; * p < 0.01, and ** p < 0.005. Abbreviations: e, eye; da, dorsal aorta; dlav, dorsal longitudinal anastomotic vessel; h, heart; i, intestine; isv, intersegmental vessel; PCV, posterior cardinal vein; sb, swim bladder; and ys, yolk sac.