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. 2017 Dec 29;19(1):93. doi: 10.3390/ijms19010093

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RT-qPCR analysis of selected lipogenic genes in bC27bSPs larvae and livers of hC27bSPs larvae. The relative mRNA expression of lipid transport (A) lipid storage (B), lipogenic factor (C), and lipogenic enzyme (D) genes in C27bSPs compared with gene expression in WT fish. The qRT-PCRs were performed in triplicate. Expression analysis of the selected genes using cDNA prepared from average four months male–female fish pairs per groups. Values were normalized against β-actin. The asterisk represents statistically significant differences; * p < 0 .01, and ** p < 0.005 levels as compared to control.