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. 2017 Dec 29;19(1):103. doi: 10.3390/ijms19010103

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Solute carrier family 9 isoform 3 (SLC9A3) expression in the murine testis (A) SLC9A3 expression in the intestines, epididymides, vas deferentia, and testes of adult wild-type mice was compared with that in the intestines of Slc9a3^−/− mice through western blotting. The upper image shows SLC9A3 expression (arrow), and the lower image shows Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression, which was used as a loading control; (B) Testis of WT mouse was detected through immunofluorescence staining with anti-SLC9A3 primary antibodies (SLC9A3 signals; green) (a). (b) Lectin (acrosome marker; red) and (c) 4′,6-diamidino-2-phenylindole (DAPI) (nucleus marker; blue) were costained and are displayed as a merged image (d). Scale bar = 50 μm.