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. 2018 Jan 2;19(1):127. doi: 10.3390/ijms19010127

Figure 4.

Figure 4

TMZ-resistant cells exhibited a lower sensitivity to TNF-α-induced VCAM-1 expression. (A) Parental U251 and TMZ-resistant cells were treated with TNF-α (10 ng/mL) for indicated time periods (0, 12, 24 h) followed by incubation with BCECF-AM-labeled-THP-1 for 30 min. The monocyte-binding ability was examined by the fluorescence microscopy and the one-way ANOVA analysis of variance was performed. The data are presented as mean ± SEM of three independent experiments. * p < 0.05 compared with the control group. (B) U251 and TMZ-resistant cells were treated with various concentrations of TNF-α (5 or 10 ng/mL) for 24 h and the expression of cell surface VCAM-1 was determined by using flow cytometry. (C) Cells were treated with various concentrations (5 or 10 ng/mL) of TNF-α for 24 h. VCAM-1 and Cx43 expressions were determined using Western blot analysis.