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. 2018 Jan 4;19(1):143. doi: 10.3390/ijms19010143

Figure 4.

Figure 4

The effect of different amount of MSG in G broth on GABA production. (A) Bacterial cells were grown in 500 mL G broth with different concentrations of MSG for 96 h at 30 °C. G broth contains 1% glucose, 2.5% yeast extract, 2 ppm calcium carbonate, 2 ppm manganese sulfate, 2 ppm Tween 80 and 10 uM PLP. Different lowercase letters indicate statistically significant differences of GABA production. (B) Bacterial cells were grown in 2 L G broth with 650 mM MSG (GM broth) in 3 L flasks at 30 °C for 112 h. Data are expressed as mean ± SD from triplicate experiments. Different letters at the top of the bars are significantly different (p < 0.05).