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. 2018 Jan 5;19(1):155. doi: 10.3390/ijms19010155

Figure 2.

Figure 2

Immunoblot analysis of LHCSR3 accumulation in transformed lines compared to WT (4A+) strain. (a,b): immunoblot analysis for strains grown in low light (a) or high light (b) using specific antibodies recognizing LHCSR3 and CP43 as a control for protein loading. Proteins extracted from WT and transformed lines were loaded at three different dilutions as reported in the figure. The phosphorylated form of LHCSR3 is indicated by *; (c–f): accumulation of LHCSR3 in low light and high light treated cells determined by densitometric analysis of filters reported in (a,b) respectively. LHCSR3 content was normalized to the chlorophyll (Chl) content (c,d) or to CP43 content (e,f). The amount of LHCSR3/Chl and LHCSR3/CP43 ratios were then normalized the ratios detected in WT cells grown in low light conditions. In the case of WT grown in high light conditions, LHCS3/Chl and LHCSR/CP43 ratios were normalized to the levels detected for WT cells grown in low light according to results reported in Figure S2. Values in (c, d, e, f) are indicated as arbitrary units (a.u.) with standard deviation reported as error bar (n = 3).